Acute toxicity and pharmacological actions of rosolic acid.

Acute toxicity and pharmacological actions of rosolic acid were studied. Results: ( 1) LD 50 s of rosolic acid on mice were 82 ( 78-87) mg/kg s.c. and 49 (41-58) mg/kg i.p. The animals survived after the oral administration. (2) Significant irritation on the mucosa was not observed. (3) Rosolic acid produced a rise in the blood pressure and a transient depression of respiration in the rabbit. ( 4) Rosolic acid increased the contractile force and heart rate in the isolated atria of the guinea pig. ( 5) Rosolic acid caused a contraction followed by a relaxation in the isolated rabbit and guinea pig ileum. INTRODUCTION The irrigation smear method introduced by Davis 1l is supposed to be a useful cytologic screening procedure for cervical cancer detection. Procuring the specimen is performed without special technique using a plastic pipette containing an irrigating and cell preservative solution. Diagnostic accuracy of more than 95 per cent was reported by this method 2l 3l 4l. Kawashima et a/.5) 6) employed rosolic acid as a pH indicator in the irrigating solution*. The present study was designed to examine acute toxicity and some pharmacological actions of rosolic acid. MATERIALS AND METHODS 1) Acute toxicity Acute toxicities were determined by the intraperitoneal, subcutaneous and oral routes in male and female mice weighing 20-24 g (SMA strain) in groups of eight. The animals were kept in an ambient temperature of 20°C and observed for 24 hours. Each group was administered with one of a logarithmic series of doses of rosolic acid, and from the mortalities observed the LD 50s and limits of error were calculated. 0.25% and 0.5% solutions of rosolic acid in 0.65% saline containing 0.2% NaOH were injected to the mice. For the ~m ~. *~ ~. ~mm~. ~*uz~. ~~~~. M~ ~ Received for publication June 6, 1969. * SSP solution®